Experienced RA-FPLC and cell culture
At Rejuvenate Bio, we aspire to treat aging and age-related diseases. We are a spinout from George Church's lab at Harvard Medical School and the Wyss Institute, and our goal is to treat pets with targeted gene therapies directed at curing specific disease states associated with old age that also have the potential to extend overall health and maximal lifespan. So, come join Rejuvenate and save all the puppies!!!!!!! (Puppy parties as celebration events are highly probable)
Duties & Responsibilities
Rejuvenate Bio is looking for a highly motivated individual to join our energetic team and accelerate the development of our therapies. The research assistant position will directly support efforts to drive forward our future commercial offerings working with other RA's and scientists. The applicant will be performing standard molecular biology procedures as well as more advanced cell culture and virus creation. The role reports to senior scientists at Rejuvenate Bio.
Responsibilities will include:
●Following laboratory protocols and safety regulations.
●Performing standard cloning techniques (restriction enzyme, gateway, goldengate)
●Performing basic and advanced cell culture on a variety of cell lines
●Performing AKTA FPLC virus and protein purification runs
●Executing experiments,independently and in conjunction with a small team in support of research studies
●Helping with AAV and Lenti-virus production
●Learning assay development techniques and procedures.
●Performing related laboratory maintenance,such as ordering supplies,managing inventory, and documenting laboratory conditions.
●Performing detailed and accurate documentation of all experiments and laboratory practices.
●Ensuring maintenance of industry-quality records to provide documentation
●Accountable for on-time, high quality results and clear communication with supervisor
B.S or M.S.in biology, biochemistry, microbiology, biomedical engineering or related field required. At least one year of FPLC or HPLC experience. At least one year of cell culture experience (splitting cells, transfection, etc) after undergrad
Experience with basic cloning techniques (restriction enzyme, gibson, golden gate, or gateway) or related experience. Experience inflow cytometry, ELISAs, PCR, and microscopy. Experience with in vitro analytical techniques to assess the cell viability of tissue constructs. Experience working with AAV. Willingness to work outside of his/her discipline to accomplish project goals. Proven ability to draft and implement lab protocols. Ability to work independently as well as in a collaborative team environment. Strong work ethic, communication skills and goal-orientedpersonality.